Quantitative RT-PCR and Western Blotting

RT–qPCR and western blotting were performed as described previously^{30 (link)}. Briefly, total RNA was isolated from liver tissues using the RNeasy® Mini Kit (QIAGEN, Hilden, Germany). RNA was reverse-transcribed using a SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific, CA, USA). RT–qPCR was performed using TaqMan Master Mix (Thermo Fisher Scientific, CA, USA) and a 7900 Sequence Detection System (Applied Biosystems, CA, USA). The specific primers used are listed in Supplemental Table 1. The antibodies used for the immunoblot are listed in Supplemental Table 2. Membranes were imaged with an Image Quant LAS 4000 camera system (GE Healthcare, IL, USA). The band intensity was quantified by ImageJ software.