Soybean variety “Williams 82” was used for quantitative PCR (qPCR) analysis. Soybean seeds were sown into flowerpots and after 2 weeks of growth, soybean seedlings were irrigated with 200 mM NaCl and 250 mM mannitol, respectively. A total of 0.1 g of soybean leaf blade tissue was collected at 0, 2, 4, 6, 8, 10, and 12 h after the 200 mM NaCl induced salt stress and 250 mM mannitol induced drought stress treatments, and total RNA was extracted using an RNA extraction kit (ZOMANBIO, ZP405, China). Double stranded cDNA was obtained using a cDNA synthesis kit (TIANGEN, KR118, China) (Le et al., 2011 (link)). qPCR reactions were performed on the ABI Prism 7500 real-time PCR system (Applied Biosystems, Foster City, CA, United States); the Actin (100500082) gene was used as the internal control (Jian et al., 2008 (link)). The expression levels of nine GmPP2A-B′′ genes were determined using the 2–ΔΔCT method. All primers are listed in Supplementary Table 2.
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