Boronic acid (BA) solution was prepared by dissolving 12 mg of 3-Aminophenylboronic acid (Sigma-Aldrich, USA) in 3 ml of dimethyl sulfoxide and 3 ml of sterile distilled water was added to this solution (14 (link)). A total of 20 μl (400 μg) of the BA solution was dispensed onto disks containing cefoxitin (FOX). A test strain was inoculated on Mueller Hinton agar (MHA) plates according to the CLSI guideline. Disks containing cefoxitin (FOX) and cefoxitin plus BA (FOX/BA) were placed on the MHA plate and incubated at 37°C overnight. An increase in the zone size of ≥5 mm for cefoxitin in the presence of BA compared with that of cefoxitin alone was considered as positive result (20 (link), 21 (link)).
Protocol detail
Boronic Acid Disk Diffusion Assay for Cefoxitin Resistance
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Bushehr University of Medical Sciences
Top 5 similar protocols
Variable analysis
independent variables
- Boronic acid (BA) solution
- Increase in the zone size of ≥5 mm for cefoxitin in the presence of BA compared with that of cefoxitin alone
- Cefoxitin (FOX) disks
- Mueller Hinton agar (MHA) plates
- Incubation at 37°C overnight
- Cefoxitin (FOX) disks
- Cefoxitin plus BA (FOX/BA) disks
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!