Morphological Analysis of K-NLCs by TEM

Morphologic analysis of K-NLCs was carried out by transmission electron microscopy (TEM) on a TEM CM12 PHILIPS equipped with an OLYMPUS Megaview G2 camera at an accelerating voltage of 80 keV [41 (link)]. For the analyses, freeze-dried K-NLCs were dispersed in ultrapure water at the initial concentration and obtained nanoparticle dispersion was placed on a carbon film-covered copper grid. Subsequently, the excess of the sample was blotted from the grid with filter paper to obtain a thin film stained with a phosphotungstic acid solution (1% w/v) in distilled water. The analysis of the samples was performed 3 min after the staining.