Senolytic Cocktail Administration in Mice

Mice were administered a senolytic cocktail containing 5 mg/kg dasatinib (D‐3307, LC Labs) and 50 mg/kg quercetin (Q4951, Sigma‐Aldrich) as described by Xu et al. (Xu et al., 2018 (link)). Briefly, 7.5 mg of dasatinib and 75 mg of quercetin were dissolved in 5 ml of 10% polyethylene glycol 400 (PEG 400; 202398, Sigma‐Aldrich). We chose this volume of PEG 400 because it allowed us to gavage a 30–45 gram mouse with 100–150 μl of D+Q, which is less than the approximate stomach volume of 400 μl in adult mice (McConnell et al., 2008 (link)). Mice were gavaged bi‐weekly for 4 months (Figure 1a) with D+Q (n = 20 young and n = 19 old) or vehicle (n = 20 young and n = 19 old; 10% PEG 400) using 20‐gauge disposable polypropylene feeding tubes (FTP‐20‐30, Instech, Plymouth Meeting, PA). These mice were then divided into 3 groups of n = 6–7/group.