The rabbit polyclonal anti-mPRβ antibody (bs-11410R; Bioss Inc) was used to immune-precipitate mPRβ. TrkA was immunoprecipitated using the rabbit polyclonal anti-TrkA antibody (#2505; CST) as described previously2 (link). To detect Rac-1 (Rac-1-GTP) in cell lysates, we used a Rac-1/Cdc-42 Activation Assay Kit (17-441, Millipore), using the manufacturer’s instructions. Cells were washed three times with ice-cold PBS and collected by gently scraping using 1 mL of ice-cold MLB Buffer (25 mM HEPES, pH 7.5, 150 mM NaCl, 1% Igepal CA-630, 10 mM MgCl2, 1 mM EDTA and 10% glycerol, aprotinin 10 μg/ml).
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