Serum, lung, liver, and kidneys were harvested from Cl-13-infected mice. Serum was isolated from whole blood collected in heparinized tubes (Fisher; Pittsburgh, PA), capped (Lecia Microsystems; Richmond, IL) and spun at 2500 rpm for 30 min. Serum was collected and snap-frozen in liquid nitrogen prior to storage at -80°C. Lung, liver and kidneys were homogenized and processed for plaque assays as previously described [48 (link)].
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