Western Blot Analysis Protocol

Preparation of cell lysates, protein collection and concentration, electrophoresis, SDS-PAGE, and immunoblotting were performed as described preciously [25 (link)]. Primary and secondary antibodies were diluted 1000-fold and 500-fold, respectively. Signals were visualized using an EZ-Western Lumi Femto (DoGenBio, Seoul, Korea) and quantified using a LAS-4000 (GE Healthcare Life Sciences, Marlborough, MA, USA) [26 (link)]. Protein band intensities on each blot were quantified by densitometric analysis using ImageJ 1.48s software (Bethesda, MD, USA).

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Chei S., Oh H.J., Lee K., Jin H., Lee J.Y, & Lee B.Y. (2020). Dietary Silk Peptide Inhibits LPS-Induced Inflammatory Responses by Modulating Toll-Like Receptor 4 (TLR4) Signaling. Biomolecules, 10(5), 771.

Publication 2020

EZ-Western Lumi Femto LAS-4000

Antibodies Cell Densitometric Electrophoresis Sds page

Corresponding Organization : CHA University

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Variable analysis

independent variables

Preparation of cell lysates
Protein collection and concentration
Electrophoresis
SDS-PAGE
Immunoblotting

dependent variables

Protein band intensities

control variables

Primary and secondary antibody dilutions

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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