For osteogenic differentiation, passage 2 AT-MSCs were seeded on 6-well plates (5.0 × 103 cells/cm2) and incubated in H-DMEM supplemented with 10% FBS and 1% antibiotic-antimycotic solution for 24 h. The medium was then changed to osteogenic medium (Cell Applications) [10 (link)]. The medium was changed twice weekly. For osteogenic analysis, mineral deposits were quantitatively analyzed by von Kossa staining after 21 days.
For adipogenic differentiation, passage 2 AT-MSCs were seeded on 6-well plates (8 × 103 cells/cm2) and cultured in H-DMEM supplemented with 10% FBS and 1% antibiotic-antimycotic solution until confluency. Then, the medium was changed to canine adipocyte differentiation medium (Cell Applications). The medium was changed twice weekly. Adipogenesis was analyzed by Oil Red O staining after 21 days.
For adipogenic differentiation, passage 2 AT-MSCs were seeded on 6-well plates (8 × 103 cells/cm2) and cultured in H-DMEM supplemented with 10% FBS and 1% antibiotic-antimycotic solution until confluency. Then, the medium was changed to canine adipocyte differentiation medium (Cell Applications). The medium was changed twice weekly. Adipogenesis was analyzed by Oil Red O staining after 21 days.
Free full text: Click here
