Living cells and mice were imaged 0, 4, 8, 24, and 48 h after cellular treatments and 0 h, 4 h, and 12–14 days after pleural delivery of pNGL-expressing cells on a Xenogen Lumina II (Perkin-Elmer, Waltham, MA) after addition of 300 μg/mL D-luciferin to culture media or isoflurane anesthesia and delivery of 1 mg intravenous D-luciferin to the retro-orbital veins8 (link)–11 (link),16 (link),25 (link),34 (link),35 (link). Data were analyzed using Living Image v.4.2 (Perkin-Elmer).
Other organizations :
Vanderbilt University, Tokyo University of Science, Ludwig-Maximilians-Universität München, Helmholtz Zentrum München, German Center for Lung Research
Imaging of living cells and mice at 0, 4, 8, 24, and 48 h after cellular treatments
Imaging of living cells and mice at 0 h, 4 h, and 12–14 days after pleural delivery of pNGL-expressing cells
control variables
Xenogen Lumina II (Perkin-Elmer, Waltham, MA) for imaging
Addition of 300 μg/mL D-luciferin to culture media or isoflurane anesthesia and delivery of 1 mg intravenous D-luciferin to the retro-orbital veins for imaging
controls
Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
