Isolation and Characterization of Lymphocytes from Bone Marrow and Spleen
Corresponding Organization :
Other organizations : University of Pennsylvania, University of California, Davis, Yale University
Variable analysis
- Isolation protocol for lymphocytes from cranial bone marrow
- Isolation protocol for splenocytes
- Expression of CD3
- Expression of MuSK
- Expression of human Ig light chain lambda
- Expression of human Ig light chain kappa
- Expression of human IgG
- Expression of mouse IgG
- Sterile conditions
- Use of cell strainer (70 μm)
- Incubation time (30 min)
- Incubation temperature (on ice)
- Not explicitly mentioned
- Not explicitly mentioned
Annotations
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