Anaerobic Culture of Fusobacterium nucleatum

Fusobacterium nucleatum was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA; #25586). WT Fusobacterium nucleatum and FadA−/− Fusobacterium nucleatum were cultured in Columbia blood agar with 5 μg/mL heme, 5% desalted sheep blood, and 1 μg/mL vitamin K1 (Sigma-Aldrich, St. Louis, MO, USA) in a 37 °C anaerobic glove box containing 85% N₂, 10% H₂ and 5% CO₂ [30 (link)]. Escherichia coli (MG1655, ATCC, Manassas, VA, USA) was propagated in Luria Bertani medium (BD Biosciences, Franklin Lakes, NJ, USA) at 37 °C in an aerobic incubator.

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Guo P., Tian Z., Kong X., Yang L., Shan X., Dong B., Ding X., Jing X., Jiang C., Jiang N, & Yu Y. (2020). FadA promotes DNA damage and progression of Fusobacterium nucleatum-induced colorectal cancer through up-regulation of chk2. Journal of Experimental & Clinical Cancer Research : CR, 39, 202.

Publication 2020

Fusobacterium nucleatum vitamin K1 Escherichia coli (MG1655, Luria Bertani medium

Aerobic Agar Blood Cultured blood Escherichia coli Fusobacterium nucleatum Heme Sheep Vitamin k1

Corresponding Organization :

Other organizations : Qingdao University, Affiliated Hospital of Qingdao University

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Variable analysis

independent variables

WT Fusobacterium nucleatum
FadA−/− Fusobacterium nucleatum

dependent variables

Not explicitly mentioned

control variables

Columbia blood agar with 5 μg/mL heme, 5% desalted sheep blood, and 1 μg/mL vitamin K1
Anaerobic environment with 85% N2, 10% H2 and 5% CO2
37 °C temperature

controls

Escherichia coli (MG1655, ATCC, Manassas, VA, USA) cultured in Luria Bertani medium at 37 °C in an aerobic incubator

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