Protocol detail

Mesenchymal Stem Cell Culture Protocol

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MSCs cultures were derived from de-identified bone marrow specimens from patients at the West Virginia University Cancer Institute and cultured as previously described [1 (link)]. Patients had no history of exposure to chemotherapy, radiation, or malignancy. HS-5 and HS-27A cell lines were purchased from ATCC (Manassas, VA, USA) and cultured as suggested by the supplier.

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Hare I., Evans R., Fortney J., Moses B., Piktel D., Slone W, & Gibson L.F. (2016). Chemotherapy-induced Dkk-1 expression by primary human mesenchymal stem cells is p53 dependent. Medical oncology (Northwood, London, England), 33(10), 113.

Publication 2016

HS-27A

Bone marrow Cell lines Chemotherapy Malignancy Patients Radiation

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Patients had no history of exposure to chemotherapy, radiation, or malignancy

controls

Positive control: HS-5 and HS-27A cell lines purchased from ATCC and cultured as suggested by the supplier
Negative control: Not mentioned

Annotations

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