Mammalian expression constructs used include: Retroviral vectors encoding shRNA for luciferase-RNAi and EWS::FLI-RNAi, as well as cDNA-containing vectors encoding 3xFLAG-EF, 3xFLAG-EF DBD, 3xFLAG-EF DBD+ (Boone et al., 2021 (link)). The EWS::FLI DBD, and EWS::FLI DBD+ were ordered as gene blocks (Integrated DNA Technologies) and cloned into the pMSCV-hygro plasmid between BamHI and AgeI restriction sites.
Free full text: Click here