Surgical Induction of Myocardial Infarction in Mice
Corresponding Organization : University of Edinburgh
Variable analysis
- Ligation of the left anterior descending (LAD) coronary artery
- Successful induction of injury (assessed at day 1 post-MI)
- Regeneration (assessed at day 21 post-MI)
- Ultrasound, histologic and biochemical techniques used to assess outcomes
- Anaesthesia induced by a combination of hypothermia and breathable (isoflurane) anaesthetic
- Animal secured onto an ice pack for the duration of the procedure to maintain adequate anaesthesia
- Analgesia administered with one drop of 1/50 bupivacaine in 0.9% saline onto the wound
- The entire litter was tattooed for identification purposes, dabbed with material from the maternal cage and returned to the nest
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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