The following cytokines have been measured in gastric juice using a multiplex immunoassay (Bio-Rad Laboratories Inc., Hercules, CA, USA), with Bioplex 100 xMAP technology (Luminex Corp, Austin, TX, USA): GM-CSF, ET-1, IFN-γ, IL-1β, TNF-α, IL-2, IL-6, IL-9, IL-17, IL-21, IL-22, IL-23, IL-10, and TGF-β. Reliability of cytokines measurement at low pH was performed according to another previously published paper [19 (link)].
Data analysis was performed using the Bioplex Manager 4.1 software (Bio-Rad Laboratories, Segrate, Italy). All samples were analyzed at the same time, and every 96-well plate included an 8-point standard curve. Cytokines concentrations below the detection limit or outside the linear part of the curve were excluded from the analysis [20 (link)].
Data analysis was performed using the Bioplex Manager 4.1 software (Bio-Rad Laboratories, Segrate, Italy). All samples were analyzed at the same time, and every 96-well plate included an 8-point standard curve. Cytokines concentrations below the detection limit or outside the linear part of the curve were excluded from the analysis [20 (link)].
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