Comparative Proteomic Analysis of Cell Pellets

The cell pellets collected from 10-I, 50-I, and 50-T were used for proteomic analysis. The digested peptides were labeled using iTRAQ reagents (AB Sciex). The labeled peptides were analyzed on an EASY-nLC 1200 system coupled to a Q-Exactive mass spectrometer (Thermo-Fisher). All raw data were collected using Thermo Xcalibur and analyzed using Sequest HT (Thermo-Fisher). Proteomic data have been deposited to the ProteomeXchange Consortium via the iProx partner repository with the dataset identifier PXD013207. The prediction of transmembrane helices were conducted by TMHMM [76 (link)]. PSORTb [77 (link)] was used to predict the proteins’ subcellular localization, and SignalP [78 (link)] was used to predict the presence of signal peptides.

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He P., Duan H., Han W., Liu Y., Shao L, & Lü F. (2019). Responses of Methanosarcina barkeri to acetate stress. Biotechnology for Biofuels, 12, 289.

Publication 2019

iTRAQ reagents EASY-nLC 1200 system Q-Exactive mass spectrometer Thermo Xcalibur Sequest HT

Cell Helices Pellets Peptides Proteins Signal peptides

Corresponding Organization :

Other organizations : State Key Laboratory of Pollution Control and Resource Reuse, Tongji University

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Variable analysis

independent variables

Cell pellets collected from 10-I, 50-I, and 50-T

dependent variables

Proteomic analysis

control variables

Digested peptides were labeled using iTRAQ reagents (AB Sciex)
Labeled peptides were analyzed on an EASY-nLC 1200 system coupled to a Q-Exactive mass spectrometer (Thermo-Fisher)
All raw data were collected using Thermo Xcalibur and analyzed using Sequest HT (Thermo-Fisher)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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