Primer sequences specific to Bid, Bak, Bcl-xL, Bax, Bcl-2 and Bad genes were used to ensure targeted amplification. The expression levels of these genes were normalized against a housekeeping gene (GAPDH). The oligonucleotides of the primers used in the present study were supplied by Thermo Fisher Scientific, Inc., Waltham, MA, USA, and their sequence is presented in detail in Table 1 .
The analysis was conducted using the 2−ΔΔCt method, which allowed for the determination of fold changes in gene expression relative to untreated control cells.
The analysis was conducted using the 2−ΔΔCt method, which allowed for the determination of fold changes in gene expression relative to untreated control cells.
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