Cysteine-Appended TLR2 Targeting Peptide Conjugation

A cysteine (Cys) residue was synthetically appended to the C-terminal of the TLR2 targeting peptide, resulting in an amino acid sequence represented as His-Leu-Tyr-Val-Ser-Pro-Trp-Cys. TLR2 targeting peptide was co-incubated and conjugated to the orid-liposome-MAL. The drugs named as 1:1 and 5:1 differed only in the amount of TLR2 pep. TLR2 pep-orid-liposome 1:1 represents the molar ratio of 1:1 for oridonin: TLR2 peptide, whereas 5:1 for TLR2 pep-orid-liposome 5:1. Dialysis was performed to eliminate any excess free peptide post-incubation.

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Liu Y., Wang X., Feng H., Li X., Yang R., Zhang M., Du Y., Liu R., Luo M., Li Z., Liu B., Wang J., Wang W., An F., Niu F, & He P. (2024). Glutathione-depleting Liposome Adjuvant for Augmenting the Efficacy of a Glutathione Covalent Inhibitor Oridonin for Acute Myeloid Leukemia Therapy. Journal of Nanobiotechnology, 22, 299.

Publication 2024

Corresponding Organization : Xi'an Jiaotong University

Other organizations : First Affiliated Hospital of Xi'an Jiaotong University

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Variable analysis

independent variables

Amount of TLR2 peptide

dependent variables

Conjugation of TLR2 peptide to orid-liposome-MAL

control variables

Cysteine (Cys) residue synthetically appended to the C-terminal of the TLR2 targeting peptide
Dialysis performed to eliminate any excess free peptide post-incubation

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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