Antibody Generation and Characterization

The rabbit anti-UL21 polyclonal antibodies were generated for this study, and the rat anti-UL16 polyclonal antibodies were provided by He Qin [20 (link)]. The following monoclonal antibodies were used in this study: rabbit anti-GRP78 BiP (Abcam, UK), mouse anti-TGN46 (Abcam, UK), goat anti-rabbit IgG (Thermo Fisher Scientific, USA), rabbit anti-Myc tag (Beyotime, CHN), mouse anti-Flag tag (Transgen Biotech, CHN), Alexa Fluor 594 goat anti-rabbit IgG (Thermo Fisher Scientific, USA), Alexa Fluor 488 goat anti-mouse IgG (Thermo Fisher Scientific, USA), Alexa Fluor 488 goat anti-rat IgG (Abcam, UK), Alexa Fluor 594 goat anti-rabbit IgG (Life Technologies, USA), and mouse anti-β-actin (Beyotime, CHN). Normal rabbit IgG was obtained from Beyotime, and normal rat IgG was obtained from Thermo. The pCAGGS [40 (link)], pCMV-Myc [41 (link)], and pET-32c plasmids were provided by the Sichuan Agricultural University Avian Diseases Research Center.

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Yang L., Wang M., Zeng C., Shi Y., Cheng A., Liu M., Zhu D., Chen S., Jia R., Yang Q., Wu Y., Zhang S., Zhao X., Huang J., Liu Y., Ou X., Mao S., Yu Y., Zhang L., Tian B., Pan L., Rehman M.U, & Chen X. (2020). Duck enteritis virus UL21 is a late gene encoding a protein that interacts with pUL16. BMC Veterinary Research, 16, 8.

Publication 2020

goat anti-rabbit IgG Alexa Fluor 594 Goat anti-Rabbit IgG Alexa Fluor 488 goat anti-mouse IgG Alexa Fluor 488 goat anti-rat IgG mouse anti-β-actin Normal rabbit IgG

Actin Alexa 594 Alexa fluor 488 Anti antibodies Anti igg Avian diseases Goat Grp78 Monoclonal antibodies Mouse Plasmids Rabbit

Corresponding Organization : Sichuan Agricultural University

Top 5 similar protocols

Variable analysis

independent variables

Rabbit anti-UL21 polyclonal antibodies
Rat anti-UL16 polyclonal antibodies
Rabbit anti-GRP78 BiP (Abcam, UK)
Mouse anti-TGN46 (Abcam, UK)
Rabbit anti-Myc tag (Beyotime, CHN)
Mouse anti-Flag tag (Transgen Biotech, CHN)
Alexa Fluor 594 Goat anti-Rabbit IgG (Thermo Fisher Scientific, USA)
Alexa Fluor 488 goat anti-mouse IgG (Thermo Fisher Scientific, USA)
Alexa Fluor 488 goat anti-rat IgG (Abcam, UK)
Alexa Fluor 594 goat anti-rabbit IgG (Life Technologies, USA)
Mouse anti-β-actin (Beyotime, CHN)
PCAGGS [40 (link)]
PCMV-Myc [41 (link)]
PET-32c

dependent variables

Not explicitly mentioned

control variables

Goat anti-rabbit IgG (Thermo Fisher Scientific, USA)
Normal rabbit IgG (Beyotime)
Normal rat IgG (Thermo)

Annotations

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