Assessing Maternal Metabolic Profile

In plasma samples collected between 24th and 29th week of pregnancy, we measured glucose and insulin levels using glucose hexokinase method (Roche Diagnostics) and Human Milliplex map kits (EMD Millipore, catalog no HMHEMAG-34K), respectively,14 (link) as well as total cholesterol, high-density lipoprotein cholesterol (HDL-C) and triglyceride levels using colorimetric methods (Johnson & Johnson Clinical Diagnostics). We estimated IS with the Matsuda Index using the formula: 10 000/√((fasting glucose×fasting insulin)×(mean glucose×mean insulin during OGTT)),16 (link) with concentrations in glucose and insulin being expressed as mg/dL and µU/mL.17 (link) We excluded from the analyses participants for which data were missing for the Matsuda Index (n=15). We computed low-density lipoprotein cholesterol (LDL-C) concentration with the Friedewald’s equation.14 (link)

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Légaré C., Desgagné V., Poirier C., Thibeault K., White F., Clément A.A., Scott M.S., Jacques P.É., Perron P., Guérin R., Hivert M.F, & Bouchard L. (2022). First trimester plasma microRNAs levels predict Matsuda Index-estimated insulin sensitivity between 24th and 29th week of pregnancy. BMJ Open Diabetes Research & Care, 10(2), e002703.

Publication 2022

glucose hexokinase method Human Milliplex map kits

Cholesterol Colorimetric Diagnostics Glucose Hexokinase High density lipoprotein cholesterol Human Insulin Low density lipoprotein cholesterol Ogtt Plasma Pregnancy Triglyceride

Corresponding Organization : Centre Hospitalier Universitaire de Sherbrooke

Other organizations : Harvard University, Massachusetts General Hospital, Harvard Pilgrim Health Care

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Variable analysis

independent variables

Glucose levels
Insulin levels

dependent variables

Insulin sensitivity (Matsuda Index)
Total cholesterol
High-density lipoprotein cholesterol (HDL-C)
Triglyceride levels
Low-density lipoprotein cholesterol (LDL-C)

control variables

Plasma samples collected between 24th and 29th week of pregnancy
Glucose measured using glucose hexokinase method (Roche Diagnostics)
Insulin measured using Human Milliplex map kits (EMD Millipore, catalog no HMHEMAG-34K)
Total cholesterol, HDL-C, and triglycerides measured using colorimetric methods (Johnson & Johnson Clinical Diagnostics)
LDL-C calculated using Friedewald's equation

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