Western Blotting Protocol for Protein Analysis

Western blotting was performed as previously described [25 (link)]. The primary antibodies used for Western blotting were as follows: anti-NDFIP2(1:1000, Bioss Antibodies Inc., Beijing, China; Cat # bs-19059R), anti-pan AKT (1:1000, Abcam, Cambridge, UK; Cat # ab8805), anti- phospho-AKT1 (1:1000, Abcam, Cat # ab66138), anti-N-cadherin (1:1000, Abcam, Cat # ab18203), anti-E-cadherin (1:10,000, Abcam, Cat # ab40772). An anti-β-actin antibody (1:2000, Sigma, USA) was used as an internal control to ensure equal amounts of protein loading.

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Zhou Y., Hu J., Liu L., Yan M., Zhang Q., Song X., Lin Y., Zhu D., Wei Y., Fu Z., Hu L., Chen Y, & Li X. (2021). Gly-tRF enhances LCSC-like properties and promotes HCC cells migration by targeting NDFIP2. Cancer Cell International, 21, 502.

Publication 2021

anti-NDFIP2 anti-pan AKT anti-N-cadherin anti-E-cadherin anti-β-actin antibody

Actin Akt1 Anti antibody Antibodies Cadherin Cadherin 1 Protein

Corresponding Organization :

Other organizations : Lanzhou University, First Hospital of Lanzhou University

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Variable analysis

independent variables

Anti-NDFIP2 antibody (1:1000, Bioss Antibodies Inc., Beijing, China; Cat # bs-19059R)
Anti-pan AKT antibody (1:1000, Abcam, Cambridge, UK; Cat # ab8805)
Anti-phospho-AKT1 antibody (1:1000, Abcam, Cat # ab66138)
Anti-N-cadherin antibody (1:1000, Abcam, Cat # ab18203)
Anti-E-cadherin antibody (1:10,000, Abcam, Cat # ab40772)

dependent variables

NDFIP2 protein expression
Pan AKT protein expression
Phospho-AKT1 protein expression
N-cadherin protein expression
E-cadherin protein expression

control variables

Anti-β-actin antibody (1:2000, Sigma, USA) used as an internal control to ensure equal amounts of protein loading

Annotations

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