Transcriptomic Analysis of Lily Tepals

Total RNA was isolated from ‘Tiny Padhye’ upper and basal tepals in four stages using an RNAprep Pure Plant Kit (polysaccharide & polyphenolic-rich) (TIANGEN, Beijing, China), according to the manufacturer’s instructions. Briefly, the first-strand cDNA was synthesized using TransScript® II One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech, Beijing, China) and oligo_(dT) primers. The qRT-PCR reactions were performed using Perfectstar™ Green qPCR SuperMix (TransGen Biotech, Beijing, China) and a Bio-Rad CFX96 system. The relative expression level of quantification was calculated based on the 2^−∆∆Ct formula method [49 (link)]. LilyActin was used as an internal control [50 (link)]. The primers for RT-qPCR were synthesized by Sangon Biotech (Shanghai, China; Table S1).