Lipid-based Nanoparticle Formulation

The 1,2-distearoryl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol-2000)] ammonium salt (DSPE-PEG), dioleoyl phosphatidic acid (DOPA) and 1,2-dioleoyl-3-trimethylammonium-propane chloride salt (DOTAP) were purchased from Avanti Polar Lipids (Alabaster, AL). The DSPE-PEG-AEAA was synthesized according to previous publication of our lab.^{58 (link)} Doxorubicin, cholesterol and protamine were purchased from Sigma-Aldrich (St. Louis, MO). DiI was purchased from ThermoFisher Scientific (Waltham, MA).

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Liu Q., Zhu H., Tiruthani K., Shen L., Chen F., Gao K., Zhang X., Hou L., Wang D., Liu R, & Huang L. (2018). Nanoparticle-Mediated Trapping of Wnt Family Member 5A in Tumor Microenvironments Enhances Immunotherapy for B-Raf Proto-Oncogene-Mutant Melanoma. ACS nano, 12(2), 1250-1261.

Publication 2018

1,2-dioleoyl-3-trimethylammonium-propane chloride salt (DOTAP) DSPE-PEG Doxorubicin cholesterol protamine

Alabaster Ammonium salt Chloride salt Cholesterol Doxorubicin Dspe Lipids Phosphatidic acid Phosphoethanolamine Polyethyleneglycol 2000 Propane Protamine

Corresponding Organization :

Other organizations : Hubei University of Technology, Texas Tech University

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Variable analysis

independent variables

DSPE-PEG
DOTAP
DSPE-PEG-AEAA
Doxorubicin
Cholesterol
Protamine

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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