Detailed Lung Single-Cell Phenotyping

Flowcytometry was performed using same materials and methods as described earlier.21 (link) Pulmonary single cell suspension was prepared using Lung dissociation Kit (130‐095‐927, Miltenyi Biotec) according to manufacturer instructions. The single‐cell suspension was then used for two different staining panels targeting myeloid or lymphoid populations. Viability staining was performed using Aqua Dead Cell Stain Kit (L34965, Thermo Fisher) or Near‐IR Dead Cell Stain Kit (L34975, Thermo Fisher) according to the manufacturer's instructions, followed by 10‐minute FC blocking. Myeloid or Lymphoid antibody mixes (Table 2) were applied for 30 minutes in 4°C prior to flow cytometry analysis. Figures S6 and S7 demonstrate gating strategies used in this study.