Cigarette Smoke and Decanoic Acid Effects

The CSE was acquired as described previously^{14 (link)}. Briefly, we burned one cigarette (carbon monoxide 14 mg/cigarette, nicotine 1 mg, tar 13 mg; Tobacco Hunan Industrial, China), collected the smoke in 20 mL of EGM-2 by a vacuum pump, removed particles and bacteria by 0.22 μM pores, then diluted the solution to 1% CSE with EGM-2. In 2 mL of EGM-2, we dissolved 5 g of Dec (Sigma, California, USA), then diluted the solution to 2 μmol/L with EGM-2 and stored it at -80°C until the experiments.

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He Z.H., Chen Y., Chen P., Xie L.H., Liang G.B., Zhang H.L, & Peng H.H. (2021). Cigarette smoke extract affects methylation status and attenuates Sca-1 expression of mouse endothelial progenitor cell in vitro. Tobacco Induced Diseases, 19, 08.

Publication 2021

EGM-2

Bacteria Carbon 14 Nicotine Smoke Tobacco Vacuum

Corresponding Organization : Second Xiangya Hospital of Central South University

Other organizations : Third Xiangya Hospital, Intensive Care Society

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Variable analysis

independent variables

Concentration of cigarette smoke extract (CSE)

dependent variables

Not explicitly mentioned

control variables

Volume of EGM-2 used to dissolve cigarette smoke and Dec
Pore size (0.22 μM) used to remove particles and bacteria from the cigarette smoke
Concentration of Dec (2 μmol/L)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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