Quantifying Total Antioxidant Capacity in Oocytes

The total antioxidant capacity in the oocytes were analyzed using Total Antioxidant Capacity Assay Kit with ABTS method (Beyotime, Beijing, China) according to the manufacturer’s protocol. The ABTS test measures the total antioxidant capacity of a sample and it is based on the ABTS• + radical discoloration. The cationic radical ABTS • + is a chromophore that absorbs at a wavelength of 734 nm and is generated by an oxidation reaction of ABTS (2,2ʹ-azino-bis- (3-ethylbenzthiazolin-6-ammonium sulfonate) with potassium persulfate.13 (link)

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Huang K., Li C., Gao F., Fan Y., Zeng F., Meng L., Li L., Zhang S, & Wei H. (2021). Epigallocatechin-3-Gallate Promotes the in vitro Maturation and Embryo Development Following IVF of Porcine Oocytes. Drug Design, Development and Therapy, 15, 1013-1020.

Publication 2021

Total Antioxidant Capacity Assay Kit with ABTS method

Abts Ammonium Antioxidant Assay Cationic Oocytes Potassium persulfate Sulfonate

Corresponding Organization : South China Agricultural University

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independent variables

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dependent variables

Total antioxidant capacity in the oocytes

control variables

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controls

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Negative control: Not mentioned

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