Gastrointestinal (GI) transit time was assessed immediately following the fourth oral challenge with intragastric OVA, on day 24 after allergic sensitization at ZT3. Mice were gavaged with a 0.25 mL solution with 6% carmine red (C1022, Sigma-Aldrich), 0.5% methylcellulose (M0512, Sigma-Aldrich), and 40 mg grade III OVA. After oral gavage and for the duration of the assay, mice were individually placed in clean cages containing regular bedding. Mice had free access to food and water and were monitored for the occurrence of diarrhoea. The GI transit time was measured as the time between oral gavage and the appearance of the first faecal pellet containing the red carmine dye. Mice were grouped together at the end of the assay.
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Gastrointestinal Transit Time in Mice
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Corresponding Organization : Howard Hughes Medical Institute
Other organizations : Délégation Paris 7, Sorbonne Paris Cité, Université Paris Cité, Inserm, Canadian Nautical Research Society
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Variable analysis
independent variables
- Oral challenge with intragastric OVA
- Gastrointestinal (GI) transit time
- Occurrence of diarrhoea
- Mice were individually placed in clean cages containing regular bedding
- Mice had free access to food and water
- Positive control: None specified
- Negative control: None specified
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