Quantification of Inflammatory Markers

Serum levels of IL-1β and TNF-α were determined using a high sensitivity ELISA kit (catalogue no. 50–112-9749 for IL-1β) and (catalogue no. 50–112-8954 for TNF-α) from eBioscience (Fisher Scientific, Waltham, MA, USA), according to manufacturer instructions and as described previously by our group [25 (link)]. Inflammatory mediators were expressed as pg/ml and normalised to protein concentration in each sample.

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Elshaer S.L., Alwhaibi A., Mohamed R., Lemtalsi T., Coucha M., Longo F.M, & El-Remessy A.B. (2019). Modulation of the p75 neurotrophin receptor using LM11A-31 prevents diabetes-induced retinal vascular permeability in mice via inhibition of inflammation and the RhoA kinase pathway. Diabetologia, 62(8), 1488-1500.

Publication 2019

high sensitivity ELISA kit

Elisa Il 1β Inflammatory mediators No 112 Protein Sensitivity Serum Tnf α

Corresponding Organization :

Other organizations : Stanford University, Charlie Norwood VA Medical Center

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum levels of IL-1β
Serum levels of TNF-α

control variables

Protein concentration in each sample

controls

None explicitly mentioned

Annotations

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