Antibodies for Immunofluorescence and Western Blot

Antibodies used for analysis by immunofluorescence or western blot were: mouse monoclonal anti-acetylated tubulin (Sigma-Aldrich); rabbit polyclonal anti-BiP (Cell Signaling); rabbit polyclonal anti-calnexin (Enzo Life Science); mouse monoclonal anti-Flag (Sigma-Aldrich); mouse monoclonal anti-myc (Santa Cruz); rabbit polyclonal anti-myc (Sigma-Aldrich); guinea pig polyclonal anti-PLIN2 [Progen); rabbit polyclonal against PLIN3 [38 (link)]; rabbit polyclonal anti-REEP5 (Proteintech); mouse monoclonal anti-spastin (6C6) (Sigma-Aldrich); rabbit polyclonal against residues 87–354 of human spastin [64 (link)].

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Papadopoulos C., Orso G., Mancuso G., Herholz M., Gumeni S., Tadepalle N., Jüngst C., Tzschichholz A., Schauss A., Höning S., Trifunovic A., Daga A, & Rugarli E.I. (2015). Spastin Binds to Lipid Droplets and Affects Lipid Metabolism. PLoS Genetics, 11(4), e1005149.

Publication 2015

mouse monoclonal anti-acetylated tubulin anti-BiP mouse monoclonal anti-Flag mouse monoclonal anti-myc mouse monoclonal anti-spastin (6C6)

Antibodies Calnexin Guinea pig Human Immunofluorescence Mouse Progen Rabbit Spastin Tubulin Western blot

Corresponding Organization : University of Cologne

Other organizations : IRCCS Eugenio Medea

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Variable analysis

independent variables

Mouse monoclonal anti-acetylated tubulin (Sigma-Aldrich)
Rabbit polyclonal anti-BiP (Cell Signaling)
Rabbit polyclonal anti-calnexin (Enzo Life Science)
Mouse monoclonal anti-Flag (Sigma-Aldrich)
Mouse monoclonal anti-myc (Santa Cruz)
Rabbit polyclonal anti-myc (Sigma-Aldrich)
Guinea pig polyclonal anti-PLIN2 (Progen)
Rabbit polyclonal against PLIN3 (link provided)
Rabbit polyclonal anti-REEP5 (Proteintech)
Mouse monoclonal anti-spastin (6C6) (Sigma-Aldrich)
Rabbit polyclonal against residues 87–354 of human spastin (link provided)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

Annotations

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