Two to three weeks after tumor cell transplantation, tumor load in blood (defined as the number of CD5+CD19+ CLL cells/L) was measured, and mice were assigned to different treatment arms to achieve comparable tumor load in all groups at baseline prior to treatment. Ibrutinib (provided by Pharmacyclics LLC, an AbbVie Company) was administered in drinking water containing sterile control vehicle (1% HP--CD) at a concentration of 0.16 mg/mL, as previously described.26 (link) For PD-1/PD-L1 blockade, mice were injected i.p. with 0.2 mg of PD-1 (clone: RMP1-14), PD-L1 (clone: 10F.9G2), or rat IgG2a isotype control antibody (clone: 2A3; all from BioXcell, West Lebanon, NH, USA) every 3 days for 4 weeks.
Protocol detail
Ibrutinib and PD-1/PD-L1 Blockade in CLL
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Antibody
Arms
Blood
Cell
Cell transplantation
Cells l
Clone
Ibrutinib
Igg2a
Isotype
Mice
Pd 1 pd l1 blockade
Pd l1
Sterile
Tumor
Tumor load
Corresponding Organization :
Other organizations : Heidelberg University, German Cancer Research Center, Universität Ulm
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Variable analysis
independent variables
- Ibrutinib administration in drinking water at a concentration of 0.16 mg/mL
- PD-1 antibody (clone: RMP1-14) injection i.p. every 3 days for 4 weeks
- PD-L1 antibody (clone: 10F.9G2) injection i.p. every 3 days for 4 weeks
- Tumor load in blood (defined as the number of CD5+CD19+ CLL cells/L)
- Tumor load in blood was measured two to three weeks after tumor cell transplantation and mice were assigned to different treatment arms to achieve comparable tumor load in all groups at baseline prior to treatment
- Sterile control vehicle (1% HP-CD) in drinking water
- Rat IgG2a isotype control antibody (clone: 2A3) injection i.p. every 3 days for 4 weeks
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