Tracing Adipocyte Lineage in Mice

Male congenic C57BL/6J mice hemizygous for a Fatty Acid Binding Protein 4 (Fabp4)-Cre transgene (35 (link)) were mated to homozygous R26R-EYFP females (36 (link)). The +/Fabp4-Cre;+/R26R-EYFP and ^+/+;+/R26R-EYFP (control) male offspring from these matings were genotyped as previously described (30 (link)). Freshly dissected organs were visualized with a Leica MZFLIII microscope (Wetzlar, Germany) and an epifluorescent yellow fluorescent protein (YFP) filter. Photographs were taken with a CoolSNAP camera and PMCapture Pro 6.0 software (Photometrics, Tucson, AZ, USA).

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O’Hara L., McInnes K., Simitsidellis I., Morgan S., Atanassova N., Slowikowska-Hilczer J., Kula K., Szarras-Czapnik M., Milne L., Mitchell R.T, & Smith L.B. (2014). Autocrine androgen action is essential for Leydig cell maturation and function, and protects against late-onset Leydig cell apoptosis in both mice and men. The FASEB Journal, 29(3), 894-910.

Publication 2014

MZFLIII microscope

Congenic mice Fabp4 Females Hemizygous Homozygous Male Microscope Protein Transgene

Corresponding Organization :

Other organizations : Bulgarian Academy of Sciences, Queen's University, Children's Memorial Health Institute

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Variable analysis

independent variables

Genotype ('+/Fabp4-Cre;+/R26R-EYFP' vs '^+/+;+/R26R-EYFP (control)')

dependent variables

Expression of yellow fluorescent protein (YFP) in freshly dissected organs

control variables

Male C57BL/6J mice
Homozygous R26R-EYFP females

controls

The '^+/+;+/R26R-EYFP' genotype is used as a control group.

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