Antibody Sources for Cell Biology

Rabbit polyclonal antibodies to human SUN1 and SUN2 have been previously described (Turgay et al., 2010 (link); Sosa et al., 2012 (link)). Antibodies against GFP and human full-length NudE were generated in rabbits and affinity purified. The mouse monoclonal antibodies against α-tubulin (T5168) and β-actin (A1978) were purchased from Sigma-Aldrich. The rabbit polyclonal antibodies against BICD2 (ab73286), pericentrin (ab4448), and NudEL (ab25959) were purchased from Abcam. Rabbit polyclonal anti–β-actin (A2066) was obtained from Sigma-Aldrich, and mouse monoclonal anti–dynein intermediate chain (DIC; ab23905) was obtained from Abcam.

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Turgay Y., Champion L., Balazs C., Held M., Toso A., Gerlich D.W., Meraldi P, & Kutay U. (2014). SUN proteins facilitate the removal of membranes from chromatin during nuclear envelope breakdown. The Journal of Cell Biology, 204(7), 1099-1109.

Publication 2014

T5168) ab4448 A2066 ab23905

Actin Antibodies Dynein intermediate chain Human Monoclonal antibodies Mouse Nude Pericentrin Rabbit Rabbits α tubulin

Corresponding Organization : École Polytechnique Fédérale de Lausanne

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Variable analysis

independent variables

Rabbit polyclonal antibodies to human SUN1 and SUN2
Antibodies against GFP and human full-length NudE generated in rabbits and affinity purified
Mouse monoclonal antibodies against α-tubulin (T5168) and β-actin (A1978) purchased from Sigma-Aldrich
Rabbit polyclonal antibodies against BICD2 (ab73286), pericentrin (ab4448), and NudEL (ab25959) purchased from Abcam
Rabbit polyclonal anti–β-actin (A2066) obtained from Sigma-Aldrich
Mouse monoclonal anti–dynein intermediate chain (DIC; ab23905) obtained from Abcam

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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