10-week-old male FVB mice were injected i.p. with vehicle or 250 μg/kg TNF-α (n = 8 per group) and tissue was collected 24 h later. Immunohistochemical staining of Iba1 protein in the dentate gyrus of the hippocampus was performed using a rabbit polyclonal anti-Iba1 primary antibody (1 : 500, Wako Chemicals) and a fluorescent Alexa 555 goat anti-rabbit secondary antibody (1 : 500, Invitrogen), as previously described [27 (link)].
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