Human SK-MEL-3 and murine B16F10 melanoma cell lines were purchased from the American Type Culture Collection (ATCC). SK-MEL-3 cells were maintained as monolayer using culture medium consisting of McCOY’S 5A medium (Invitrogen, Cergy Pontoise, France) supplemented with 15% fetal calf serum (FCS) (Eurobio, Les Ulis, France), and 4 µg µL−1 gentamycin (Invitrogen) at 37 °C in a humidified incubator containing 5% CO2. B16F10 cells were maintained as monolayer using culture medium consisting of DMEM-Glutamax medium (Invitrogen) supplemented with 10% FCS (Eurobio), and 4 µg µL−1 gentamycin (Invitrogen) at 37 °C in a humidified incubator containing 5% CO2. The NRASQ61K 1007 (also named NRAS 1007) murine cell line was cultured as previously described [28 (link)]. Melanoma spheroids were generated as previously described [24 (link)].
Spheroids were harvested between 1 and 72 h post-[131I]ICF01012 removal, frozen in N2, and stored at −80 °C during the radioactive decay (80 days; 10 times the half-life of the iodine-131 isotope) for Western blot analysis or fixed in 70% ethanol for cell-cycle studies.
Spheroids were harvested between 1 and 72 h post-[131I]ICF01012 removal, frozen in N2, and stored at −80 °C during the radioactive decay (80 days; 10 times the half-life of the iodine-131 isotope) for Western blot analysis or fixed in 70% ethanol for cell-cycle studies.
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