Wound-Healing Assay for Cancer Cell Migration

A wound-healing assay was performed to examine the capability of cancer cell migration, as previously described^{40 (link)}. Briefly, GC cells were grown in 24-well plates with RPMI-1640 medium supplemented with 10% FBS up to 90% confluence. A single scratch wound was generated with a 10 μL pipette tip. After removing the suspension cells by washing with PBS, fresh RPMI-1640 medium without FBS was added. With a Live Cell Imaging System (ZEISS, Germany), moving and growing of cells across the scratched lines were monitored every hour for 24 h. The migratory ability of the cells was presented as the gap distance recovered compared with the original gap.