Whole-Blood Nucleic Acid Extraction and qPCR

Nucleic acids from 100 μl whole-blood samples stored in RNAprotect Cell Reagent were extracted using MagNAPure LC automatic extractor (Total Nucleic Acid Isolation Kit—High Performance, Roche Applied Science) and used for qPCR targeting
18S rRNA
^{41 (link)}. Genomic DNA from the same extraction was used to test for human haemoglobinopathies haemoglobin S and C
^{42 (link)}.

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Barry A., Behet M.C., Nébié I., Lanke K., Grignard L., Ouedraogo A., Soulama I., Drakeley C., Sauerwein R., Bolscher J.M., Dechering K.J., Bousema T., Tiono A.B, & Gonçalves B.P. (2019). Functional antibodies against Plasmodium falciparum sporozoites are associated with a longer time to qPCR-detected infection among schoolchildren in Burkina Faso. Wellcome Open Research, 3, 159.

Publication 2018

Total Nucleic Acid Isolation Kit

Blood Cell Genomic Haemoglobin s Haemoglobinopathies Human Isolation Nucleic acid

Corresponding Organization : London School of Hygiene & Tropical Medicine

Other organizations : Centre National de Recherche et de Formation sur le Paludisme, TropIQ Health Sciences

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Variable analysis

independent variables

Whole-blood samples stored in RNAprotect Cell Reagent

dependent variables

Nucleic acids extracted using MagNAPure LC automatic extractor
Quantitative PCR (qPCR) targeting 18S rRNA
Genomic DNA testing for human haemoglobinopathies haemoglobin S and C

control variables

Volume of whole-blood samples (100 μl)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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