Cellular Immunity Measurement for SARS-CoV-2
Corresponding Organization :
Other organizations : University of Osijek, University of Split, Pennsylvania State University, University of New Haven, Lee College, University of Rijeka, University of Mostar, National Forensic Sciences University, University Hospital Centre Zagreb
Variable analysis
- Stimulation conditions: (1) COV-2 IGRA Blank, (2) CoV-2 IGRA Tube, and (3) CoV-2 IGRA Stim
- IFN-γ concentration in the separated plasma
- Fresh whole blood samples collected in heparinized tubes
- Incubation of the individual whole blood in the stimulation tubes for 20–24 h at 37 °C
- Positive control: CoV-2 IGRA Stim (coated with mitogen to verify if the sample contained a sufficient number of viable and functional T cells)
- Negative control: COV-2 IGRA Blank (for measuring individual IFN-γ concentrations as it contained no activating components)
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