Cellular Immunity Measurement for SARS-CoV-2

As described in our previous study, for the analysis of cellular immunity, the Quan-T-Cell SARS-CoV-2 in combination with the Quan-T-Cell ELISA (Euroimmun Medizinische Labordiagnostika, Lübeck, Germany) was used [15 (link)]. The principle of the test is a measurement of IFN-γ concentration released by activated immune cells. Fresh whole blood samples were collected in heparinized tubes and pipetted into the three stimulation tubes (Quan-T-Cell SARS-CoV-2): (1) COV-2 IGRA (interferon-gamma release assay) Blank was used for measuring individual IFN-γ concentrations as it contained no activating components; (2) CoV-2 IGRA Tube was coated with peptide components of the S1 domain of the SARS-CoV-2 spike protein; and (3) CoV-2 IGRA Stim was coated with mitogen to verify if the sample contained a sufficient number of viable and functional T cells. After incubation of the individual whole blood in the stimulation tubes for 20–24 h at 37 °C, the separated plasma was used to determine IFN-γ concentration by Quan-T-Cell ELISA.

Free full text: Click here

Primorac D., Brlek P., Pavelić E.S., Mešić J., Glavaš Weinberger D., Matišić V., Molnar V., Srića S, & Zadro R. (2023). Importance of Cellular Immunity and IFN-γ Concentration in Preventing SARS-CoV-2 Infection and Reinfection: A Cohort Study. Viruses, 15(3), 792.

Publication 2023

Quan-T-Cell SARS-CoV-2 Quan-T-Cell ELISA

Blood Cells Cellular immunity Elisa Ifn γ Interferon gamma release assay Mitogen Peptide Plasma Sars cov 2 Sars cov 2 spike protein T cell

Corresponding Organization :

Other organizations : University of Osijek, University of Split, Pennsylvania State University, University of New Haven, Lee College, University of Rijeka, University of Mostar, National Forensic Sciences University, University Hospital Centre Zagreb

Top 5 similar protocols

Variable analysis

independent variables

Stimulation conditions: (1) COV-2 IGRA Blank, (2) CoV-2 IGRA Tube, and (3) CoV-2 IGRA Stim

dependent variables

IFN-γ concentration in the separated plasma

control variables

Fresh whole blood samples collected in heparinized tubes
Incubation of the individual whole blood in the stimulation tubes for 20–24 h at 37 °C

controls

Positive control: CoV-2 IGRA Stim (coated with mitogen to verify if the sample contained a sufficient number of viable and functional T cells)
Negative control: COV-2 IGRA Blank (for measuring individual IFN-γ concentrations as it contained no activating components)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!