Methanolic Extract Chemical Profiling

The profiling of chemical constituents in the methanolic extract of strain MUSC 14 was carried out based on method described by Tan et al. [54 (link)]. Agilent Technologies 6980N was coupled with a 5979 Mass Selective Detector. The HP-5MS (5% phenyl methyl siloxane) capillary column of dimensions 30.0 m × 250 μm × 0.25 μm was used as helium gas carrier at 1 mL/min. Initial temperature of column was set at 40°C with a gradual increase of 3°C every minute until it achieved the maximum of 250°C and then stationed for an intermediate 5 minutes. MS was set to operate at 70 eV. The detected constituents were identified by comparing their mass spectral data with standard compounds from NIST 05 spectral Library.