Maternal Blood Methylation in EOPE, LOPE, IUGR

To compare the DNAm differences between clinical groups for each of our candidate genes twenty EOPE, 11 LOPE, 8 LOPE + IUGR, 10 IUGR, and 37 control cases were run on the Illumina Infinium HumanMethylation450 BeadChip (450 k) array, which interrogates >480,000 CpG sites in >20,000 genes [35 (link)]. Some of these samples were previously analyzed in the study reported by Blair et al. (2013). To compare the association between DNAm and protein levels in maternal blood, 122 placental DNA samples (750 ng) bisulfite converted using the EZ DNA Methylation kit (Zymo Research, Irvine, USA). Hybridization of samples to the array was completed as per the manufacturer’s protocol. The microarray chips were scanned by the HiScan 2000 or iScan (Illumina). Data was normalized and analyzed as per Blair et al. (2010) methods [14 (link)].

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Wilson S.L., Blair J.D., Hogg K., Langlois S., von Dadelszen P, & Robinson W.P. (2015). Placental DNA methylation at term reflects maternal serum levels of INHA and FN1, but not PAPPA, early in pregnancy. BMC Medical Genetics, 16, 111.

Publication 2015

Infinium HumanMethylation450 BeadChip (450 k) array EZ DNA Methylation kit HiScan 2000

Bisulfite Blood protein Chips Dna methylation Eope Genes Hiscan Hybridization Iugr Microarray Placental Protein

Corresponding Organization :

Other organizations : Child and Family Research Institute

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Variable analysis

independent variables

Clinical groups (EOPE, LOPE, LOPE + IUGR, IUGR, control)

dependent variables

DNA methylation (DNAm) differences between clinical groups for each of the candidate genes
Association between DNA methylation (DNAm) and protein levels in maternal blood

control variables

Sample size (20 EOPE, 11 LOPE, 8 LOPE + IUGR, 10 IUGR, 37 control cases)
Microarray platform (Illumina Infinium HumanMethylation450 BeadChip)
Bisulfite conversion (EZ DNA Methylation kit)
Hybridization and scanning (Manufacturer's protocol, HiScan 2000 or iScan)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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