Cells were collected and centrifuged (1600 rpm, 4 °C, 5 min) to obtain the supernatant. The cell supernatant was pipetted into 96-well plates coated with antibodies for 12 h in advance. The levels of serum tumor necrosis factor alpha (TNF-α), and interleukine (IL)-6 were measured using a commercial kit according to the manufacturer’s instruction (Uncoated ELISA Kit, Invitrogen, Waltham, MA, USA).
Serum amylase and inflammatory factors were detected following the steps of manufacturer’s instruction book [19 (link)].
Serum amylase and inflammatory factors were detected following the steps of manufacturer’s instruction book [19 (link)].
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