Quantifying UGT Gene Expression

qRT-PCR was performed to determine the expression patterns of UGT genes in different strains and tissues using an Applied Biosystems™ QuantStudio™ 6 Flex Real-Time PCR System. Primers were designed by Beacon Designer 7.0 (Premier Biosoft International, Palo Alto, CA, USA) and are listed in Table S3 (Supplementary Materials). A ChamQ^TM SYBR^® qPCR Master Mix (Vazyme biotech Co., Ltd., Nanjing, China) was used according to the manufacturer’s protocol. Normalization of each experimental gene expression level was conducted based on the geometric mean of two selected reference genes, G3PDH and Actin A1 [8 (link)]. The expression stability of the two reference genes was analyzed with geNorm Software [35 (link)]. Data were calculated according to the 2^−ΔΔCt method.

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Zhao J., Xu L., Sun Y., Song P, & Han Z. (2019). UDP-Glycosyltransferase Genes in the Striped Rice Stem Borer, Chilo suppressalis (Walker), and Their Contribution to Chlorantraniliprole Resistance. International Journal of Molecular Sciences, 20(5), 1064.

Publication 2019

QuantStudio™ 6 Flex Real-Time PCR System Beacon Designer 7.0 ChamQTM SYBR® qPCR Master Mix

Actin Expression genes Genes Primers Strains Tissues

Corresponding Organization : Jiangsu Academy of Agricultural Sciences

Other organizations : Jiangxi Academy of Agricultural Sciences, Institute of Botany

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Variable analysis

independent variables

Strain
Tissue

dependent variables

Expression patterns of UGT genes

control variables

G3PDH (reference gene)
Actin A1 (reference gene)

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