Evaluating Skin Responses to Cytokine Stimuli

Full-thickness human skin models (MatTek Corp., Ashland, MA, U.S.A.) (n = 4) were incubated in assay media (MatTek Corp.) supplemented with or without rh-IL-17 (R&D Systems, Minneapolis, MN, U.S.A.) 200 ng mL⁻¹, rh-IL-22 (Peprotech Inc., Rocky Hill, NJ, U.S.A.) 200 ng mL⁻¹ 200, or rh-IFN-γ (R&D Systems, Minneapolis, MN, U.S.A.) 20 ng mL⁻¹, for 2 days. On day 2, the skin models were harvested for microarray analyses. The same concentrations used for treating in vitro monolayer keratinocytes were applied for RHE, as they were proved effective in gene modulation as previously described by our group [12] (link).