Immunohistochemistry Assays for Renal Cell Carcinoma

Renal clear cell carcinoma and adjacent noncancerous renal samples were obtained by radical nephrectomy from the First Affiliated Hospital of Nanjing Medical University (Jiangsu Province Hospital) between 2005 and 2018. All diagnoses were confirmed by senior pathologists independently. Informed consent from all patients was acquired in the study. The study design and protocol were approved by the ethics committee of the First Affiliated Hospital of Nanjing Medical University (Jiangsu Province Hospital). IHC assays were performed as previously described (49 (link), 50 (link)). Briefly, the primary antibody was diluted as follows: anti-PDHB (1:100, Abcam, USA).

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Wu J., Wang S., Liu Y., Zhang T., Wang X, & Miao C. (2023). Integrated single-cell and bulk characterization of cuproptosis key regulator PDHB and association with tumor microenvironment infiltration in clear cell renal cell carcinoma. Frontiers in Immunology, 14, 1132661.

Publication 2023

anti-PDHB

Antibody Assays Diagnoses Hospital ethics committee Nephrectomy Pathologists Patients Renal Renal clear cell carcinoma

Corresponding Organization :

Other organizations : Nanjing Medical University, Jiangsu Province Hospital, Shanghai University of Traditional Chinese Medicine, Shuguang Hospital

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Variable analysis

independent variables

Renal clear cell carcinoma and adjacent noncancerous renal samples

dependent variables

PDHB protein expression (measured by IHC assay)

control variables

Patient samples were obtained from the same hospital (First Affiliated Hospital of Nanjing Medical University) between 2005 and 2018
All diagnoses were confirmed by senior pathologists independently
Informed consent from all patients was acquired
The study design and protocol were approved by the ethics committee

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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