Protocol detail

Rearing Ae. aegypti Mosquitoes with wAlbB

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All mosquito colonies were maintained at 27 °C and 70% humidity with 12-h light/dark cycles. Larvae were fed tropical fish pellets (Tetra) whilst adults had access to 5% sucrose solution ad libitum. Blood meals were provided using a Hemotek artificial blood-feeding system (Hemotek Ltd) and human blood (Scottish National Blood Bank). Damp Grade 1 filter-paper (Whatman plc) was provided as an oviposition source for egg collection. Eggs were desiccated for 5–10 days before hatching in water containing 1 g/l bovine liver powder (MP Biomedicals). The wAlbB-carrying Ae. aegypti line used in this study was generated previously through embryo microinjection^{40 (link)}.

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McNamara C.J., Ant T.H., Harvey-Samuel T., White-Cooper H., Martinez J., Alphey L, & Sinkins S.P. (2024). Transgenic expression of cif genes from Wolbachia strain wAlbB recapitulates cytoplasmic incompatibility in Aedes aegypti. Nature Communications, 15, 869.

Publication 2024

Hemotek artificial blood-feeding system human blood bovine liver powder

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Variable analysis

independent variables

Genetic modification of Ae. aegypti mosquitoes to carry the wAlbB Wolbachia strain

dependent variables

Not explicitly mentioned

control variables

Temperature (27 °C)
Humidity (70%)
Light/dark cycle (12-h)
Food for larvae (tropical fish pellets)
Food for adults (5% sucrose solution)
Blood source for adults (human blood)
Oviposition substrate (damp filter-paper)
Egg hatching conditions (water with 1 g/l bovine liver powder)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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