The picric acid test is used to detect reducing sugars. First, 1 mL of the saturated picric acid was added to 1 mL of the test honey samples, followed by 0.5 mL of 10% sodium carbonate (10%) solution. Then, the test tube was heated in a boiling water bath and the absorbance was read at 490 nm [82 (link)].
where A sample = Sample absorbance, and A standard = Standard absorbance.
where A sample = Sample absorbance, and A standard = Standard absorbance.
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