The kidney tissues were fixed in 4% paraformaldehyde solution, and embedded in paraffin. Then, the sections of paraffin-embedded kidney tissues were stained with periodic-acid-Schiff (PAS) and hematoxylin and eosin (H&E). A Digital Slide Scanner (PANNORAMIC SCAN II, 3D HISTECH) was used to scan the stained slides. The inflammatory score and fungal burden of the kidneys were assessed as previously reported [38 (link)–40 (link)].
For immunohistochemistry (IHC) assay, the renal tissues sections were stained with the indicated primary antibodies. The stained sections were scanned by a Digital Slide Scanner. The ImageJ software was used to quantify the proportion of positive cells. The primary antibodies used were as follows: SLC7A11 (xCT) (Abcam, ab307601, 1:500), GPX4 (Abcam, ab125066, 1:100), Ly-6G (Abcam, ab238132, 1:2000), F4/80 (Cell Signaling Technology, 70076T, 1:250) and CD11c (Abcam, ab219799, 1:100).
For immunohistochemistry (IHC) assay, the renal tissues sections were stained with the indicated primary antibodies. The stained sections were scanned by a Digital Slide Scanner. The ImageJ software was used to quantify the proportion of positive cells. The primary antibodies used were as follows: SLC7A11 (xCT) (Abcam, ab307601, 1:500), GPX4 (Abcam, ab125066, 1:100), Ly-6G (Abcam, ab238132, 1:2000), F4/80 (Cell Signaling Technology, 70076T, 1:250) and CD11c (Abcam, ab219799, 1:100).
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