Establishing NOTCH1-induced T-ALL Model

The NOTCH1-induced T-ALL model was established as previously decribed.^{17 (link)} Briefly, bone marrow cells were isolated from 8-week-old donor mice. Lineage negative (Lin^-) cells were enriched using a Lineage Cell Depletion Kit (Miltenyi Biotec), and pre-stimulated for 24 h in Dulbecco modified Eagle medium (Gibco) containing 20% fetal bovine serum (Gibco), 1% penicillin/streptomycin (Hyclone), 20 ng/mL murine FLT3-L, 20 ng/mL murine thrombopoietin and 100 ng/mL murine stem cell factor (PeproTech). Cells were then transduced with MigR1-ICN1 retroviruses and centrifuged in the presence of 6 Dg/mL polybrene. A second round of transduction was carried out on the next day. One million cells were then transplanted into sub-lethally (5.5 Gy) irradiated 8-week-old C57BL/6 female mice by tail vein injection.

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Guo H., Xu J., Xing P., Li Q., Wang D., Tang C., Palhais B., Roels J., Liu J., Pan S., Huang J., Liu Z., Zhu P., Taghon T., Qing G., Van Vlierberghe P, & Liu H. (2023). RNA helicase DHX15 exemplifies a unique dependency in acute leukemia. Haematologica, 108(8), 2029-2043.

Publication 2023

Lineage Cell Depletion Kit Dulbecco modified Eagle medium fetal bovine serum murine FLT3-L murine thrombopoietin murine stem cell factor

Bone marrow cells C57bl mice Cells Donor Eagle Female Fetal bovine serum Flt3 Murine Penicillin Polybrene Retroviruses Stem cell factor Streptomycin Tail Thrombopoietin Vein

Corresponding Organization :

Other organizations : Wuhan University, Henan Cancer Hospital, Zhengzhou University, Zhongnan Hospital of Wuhan University, Beijing Institute of Genomics, Chinese Academy of Sciences, Institute of Hematology & Blood Diseases Hospital, National Clinical Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Ghent University, First Affiliated Hospital Zhejiang University

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Variable analysis

independent variables

Transduction of bone marrow cells with MigR1-ICN1 retroviruses

dependent variables

Development of T-cell acute lymphoblastic leukemia (T-ALL)

control variables

Lineage negative (Lin-) bone marrow cells
Pre-stimulation of cells for 24 h in medium containing FLT3-L, thrombopoietin, and stem cell factor
Transplantation of 1 million cells into sub-lethally (5.5 Gy) irradiated 8-week-old C57BL/6 female mice

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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