PLGA Nanoparticles for ALA Delivery

ALA hydrochloride powder was obtained from Shanghai Fudan-Zhangjiang Bio-Pharmaceutical (Shanghai, People’s Republic of China [PRC]). ALA-loaded PLGA NPs (ALA PLGA NPs) were prepared using a modified double-emulsion solvent-evaporation method as previously described.8 (link) Encapsulation efficiency was 65.8%±7.2%, and ALA-loading capacity was 0.62%±0.27%. Sheep antimouse monoclonal antibody against CD4 and CD8, peroxidase-conjugated rabbit antisheep IgG, a Strept Avidin Biotin Complex kit, and 3,3′-diaminobenzidine (DAB) chromogen were obtained from Boster Biological Technology (Wuhan, PRC).

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Wang X., Shi L., Tu Q., Wang H., Zhang H., Wang P., Zhang L., Huang Z., Zhao F., Luan H, & Wang X. (2015). Treating cutaneous squamous cell carcinoma using 5-aminolevulinic acid polylactic-co-glycolic acid nanoparticle-mediated photodynamic therapy in a mouse model. International Journal of Nanomedicine, 10, 347-355.

Publication 2015

Strept Avidin Biotin Complex kit 3,3′-diaminobenzidine (DAB) chromogen

Avidin Biological Biotin Chromogen Emulsion Monoclonal antibody Peroxidase Pharmaceutical Plga Powder Rabbit Sheep Solvent

Corresponding Organization :

Other organizations : Fudan University, Fujian Normal University, Shanghai Institute of Pharmaceutical Industry

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Variable analysis

independent variables

Preparation method of ALA-loaded PLGA NPs (ALA PLGA NPs) using a modified double-emulsion solvent-evaporation method

dependent variables

Encapsulation efficiency of ALA PLGA NPs
ALA-loading capacity of ALA PLGA NPs

control variables

ALA hydrochloride powder obtained from Shanghai Fudan-Zhangjiang Bio-Pharmaceutical (Shanghai, People's Republic of China [PRC])
Sheep antimouse monoclonal antibody against CD4 and CD8
Peroxidase-conjugated rabbit antisheep IgG
Strept Avidin Biotin Complex kit
3,3′-diaminobenzidine (DAB) chromogen obtained from Boster Biological Technology (Wuhan, PRC)

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