Phylogenetic Analysis of HAK Sequences

Twenty-seven amino acid sequences of HAK from rice and 13 amino acid sequences of HAK from Arabidopsis were obtained from the rice genome database RGAP (http://rice.plantbiology.msu.edu/) and the Arabidopsis database TAIR (https://www.arabidopsis.org/), respectively. These sequences were aligned with Moso bamboo sequences using ClustalX2.1 software, and the sequence alignment results were used to construct a phylogenetic tree using MEGA11 software with the Maximum likelihood (ML) method and 1000 bootstrap replicates for evaluation.

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Guo H., Tan J., Jiao Y., Huang B., Ma R., Ramakrishnan M., Qi G, & Zhang Z. (2024). Genome-wide identification and expression analysis of the HAK/KUP/KT gene family in Moso bamboo. Frontiers in Plant Science, 15, 1331710.

Publication 2024

Corresponding Organization : Zhejiang A & F University

Other organizations : Nanjing Forestry University

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Variable analysis

independent variables

Twenty-seven amino acid sequences of HAK from rice
Thirteen amino acid sequences of HAK from Arabidopsis

dependent variables

Sequence alignment results
Phylogenetic tree constructed using the Maximum likelihood (ML) method

control variables

Moso bamboo sequences used for alignment

controls

No positive or negative controls were explicitly mentioned.

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